Complete system for the detection of deletions at AZF locus, involved in male infertility, by multiplex amplification in the regions sY84, sY86, DFFRY, DBY, sY95, sY117, sY125, sY127, sY134, sY254, sY255, ZFX/ZFY, SRY.

The method is compliant to the European Guidelines: 'EAA/EQMN best practice guidelines for molecular diagnosis of Y-chromosomal microdeletions. State of the art 2004' by Simoni M., Bakker E., Krausz C.: Int. J. Andrology, 2004, 27: 240-249.

The kit includes:

• DNA extraction system;
• Ready to use, single-dosed reagents for the multiplex amplification;
• TAQ polymerase;
• Amplificability control (amplification of ZFX/ZFY gene);
• Reference DNA control (not-deleted male DNA);
• Reagents for agarose gel electrophoresis.

Kit for the detection of the extension of deletions at AZF locus involved in male infertility by multiplex amplification in the regions located at each distal and proximal AZF locus:

•AZFa: sY82(+), sY83(-) (prox) // (dist) sY87 (-), sY88 (+)

•AZFb: sY105(+), Y6BaH34(-) (prox) // (dist) sY143 (-), sY152(+)

•AZFc: sY143(+), sY152(-) (prox) // (dist) sY157 (-), sY158 (+)

•Yq12 (eterochromatinic region, distal to AZFc): sY160(+).

The method is consistent withe the European Guidelines: "EAA/EQMN best practice guidelines for molecular diagnosis of Y-chromosomal microdeletions. State of the art2004" Simoni M., Bakker E., Krausz C. Int. J. Andrology, 2004, 27: 240-249 "Laboratory guidelines for molecular diagnosis of Y-chromosomal microdeletions", Simoni, M., Bakker, E., Eurlings, M.C.M. et al. . Int. J. Andrology. 1999, 22:292-299.

The kit includes:

• Ready to use, single-dosed reagents for the multiplex amplification;
• TAQ polymerase;
• Amplificability control (amplification of ZFX/ZFY gene);
• Reference DNA control (not-deleted male DNA);
• Reagents for high-resolution agarose gel electrophoresis.

Kit for the detection of deletions at AZF locus, involved in male infertility, by multiplex amplification in the regions sY84, sY86, DFFRY, DBY, sY95, sY117, sY125, sY127, sY134, sY254, sY255, ZFX/ZFY, SRY.

The method is compliant to the European Guidelines: 'EAA/EQMN best practice guidelines for molecular diagnosis of Y-chromosomal microdeletions. State of the art 2004' by Simoni M., Bakker E., Krausz C.: Int. J. Andrology, 2004, 27: 240-249.

The kit includes:

• Ready to use, single-dosed reagents for the multiplex amplification;
• TAQ polymerase;
• Amplificability control (amplification of ZFX/ZFY gene);
• Reference DNA control (not-deleted male DNA);
• Reagents for agarose gel electrophoresis.

Kit for the detection of the bcl-1 gene t(11,14) (q13;q32) translocation by DNA amplification of the Major Translocation Cluster (MTC) region.

The kit includes:

• Ready to use, single-dosed reagents for nested amplification;
• TAQ polymerase;
• Positive control (recombinant plasmid DNA with BCL-1 MTC rearrangement);
• Amplificability control (amplification of beta-globin gene);
• Reagents for agarose gel electrophoresis.

Kit for the detection of the bcl-2 gene t(14;18) (q32;q21) translocation by nested amplification of Major Break-point Region (MBR) and minor cluster region (mcr).

The kit includes:

• Ready to use, single-dosed reagents for nested amplification;
• TAQ polymerase;
• Positive control (recombinant plasmid BCL-2 MBR e BCL-2 mcr rearranged DNA);
• Amplificability control (amplification of beta-globin gene);
• Reagents for agarose gel electrophoresis.

Kit for the identification of the translocation t(9;22) (q34;q11) p190 variant (m-bcr trascript) involving the abl protooncogene and the bcr gene, by c-DNA amplification (nested RT-PCR) of the relevant regions of abl gene on chromosome 9 and the bcr gene on chromosome 22.

The kit includes:

• Reagents for retrotranscription;
• Ready to use, single-dosed reagents for nested amplification;
• TAQ polymerase;
  Amplificability control (amplification of beta2-microglobulin gene);
• Positive controls (for the translocation and for beta2-microglobulin);
• Reagents for agarose gel electrophoresis.

Kit for the identification of the translocation t(9;22) (q34;q11) p210 variant (p210 M-bcr trascript) involving the abl protooncogene and the bcr gene, by c-DNA amplification (nested RT-PCR) of the relevant regions of abl gene on chromosome 9 and the bcr gene on chromosome 22.

The kit includes:

• Reagents for retrotranscription;
• Ready to use, single-dosed reagents for nested amplification;
• TAQ polymerase;
  Amplificability control (amplification of beta2-microglobulin gene);
• Positive controls (for the translocation and for beta2-microglobulin);
• Reagents for agarose gel electrophoresis.

Kit for the analysis of Ig heavy chain clonality (CDR I) by DNA amplification in the variable (V), hypervariable (H-CDRI) and junction (j) regions.

The kit includes:

• Ready to use, single-dosed reagents for amplification;
• Hot-start TAQ polymerase;
• Control DNAs (monoclonal and policlonal CDR I DNA);
• Reagents for agarose gel electrophoresis.

Kit for the analysis of Ig heavy chain clonality (CDR II) by DNA amplification in the variable (V), hypervariable (H-CDR II) and junction (j) regions.

The kit includes:

• Ready to use, single-dosed reagents for amplification;
• TAQ polymerase;
• Control DNAs (monoclonal and policlonal CDR II DNA);
• Reagents for agarose gel electrophoresis.

Kit for the detection of deletions in the Duchenne and Becker muscular dystrophy gene. by DNA amplification in exons 3, 4, 6, 8, 13, 17, 19, 43, 44, 45, 47, 48, 49, 50, 51, 52, 60.

The kit includes:

• Ready to use, single-dosed reagents for multiplex amplification;
• TAQ polymerase;
• Reagents for agarose gel electrophoresis.

Kit for the analysis of clonality of V-J regions of the gamma-chain of T-cell receptor (TCR).

The kit includes:

• Ready to use, single-dosed reagents for single strand amplification;
• TAQ polymerase;
• Reference DNA (monoclonal and policlonal g-TCR DNA);
• Reagents for agarose gel electrophoresis;