High Performance Liquid Chromatographs are developed and manufactured by Institute of Chromatography ECONOVA, Ltd., Novosibirsk hi-tech company.

These science intensive instruments designed with unique possibility to perform analyses of hundreds of chemicals by one fast run: no reference materials need and the only simple complex validation procedure is applied in accordance with renewed, certified electronic data base.

"MiLiChrom® А-02" - fully portable and automated Micro-column Liquid Chromatograph is intended to use in the stationary and mobile analytical laboratories:

- No reference materials need

- The same HPLC-UV D-Base for any "MiLiChrom® A-02"

- Hundreds of chemicals by one fast HPLC procedure

- Simple complex validation procedure

- Lower analysis cost

High Performance Liquid Chromatographs are developed and manufactured by Institute of Chromatography ECONOVA, Ltd., Novosibirsk, Academpark hi-tech company.

These science intensive instruments designed with unique possibility to perform analyses of hundreds of chemicals by one fast run: no reference materials need and the only simple complex validation procedure is applied in accordance with renewed, certified electronic data base.

"MiLiChrom® А-02" - fully portable and automated Micro-column Liquid Chromatograph is intended to use in the stationary and mobile analytical laboratories:

- No reference materials need;

- The same HPLC-UV D-Base for any "MiLiChrom® А-02";

- Hundreds of chemicals by one fast HPLC procedure;

- Simple complex validation procedure;

- Lower analysis cost.

Menu of Reagents for PCR and Molecular Biology Research from BIOSAN, Ltd.:

Reagents:

2'-Deoxynucleoside-5'-triphosphates:

- commonly used substrates for DNA synthesis;

- essential components for widespread molecular biology applications such as PCR for DNA research and medical diagnostics, DNA hybridisation probes production etc.

dATP - 2'-deoxyadenosine-5'-triphosphate

dCTP - 2'-deoxycytidine-5'-triphosphate

dGTP –2'-deoxyguanosine-5'-triphosphate

TTP - thymidine-5'-triphosphate

dUTP – 2'-deoxyuridine-5'-triphosphate

All products are available as sodium, lithium, ammonium or triethylammonium salts.

Quality of dNTPs:

Purity of dNTPs is 98 – 99 % according to HPLC analysis.

All product are free of DNases, RNases and phosphatases activities.

All product are free of pyroposphate.

All product are free of DNA and RNA.

dNTPs allows for synthesis DNA PCR-products up to 20 kilobases long, Real-Time PCR and other applications.

Modified Deoxynucleotide-5'-Triphosphate from BIOSAN, Ltd.:

AA-dUTP — 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate

AA-UTP — 5-(3-aminoallyl)-2'-uridine 5'-triphosphate

AZTTP — 3'-Azido-3'-deoxythymidine-5'-triphosphate

Bio-11-dUTP — 5-[N-(N-biotinyl-Є-aminocaproyl)-3-aminoallyl]-2'-deoxyuridine-5'-triphosphate

Br-dUTP — 5-Bromo-2'-deoxyuridine-5'-triphosphate

7-deaza-dGTP — 7-deaza-2'-deoxyguanosine-5'-triphosphate

FITC-4-dUTP — Fluorescein-6-aminothiocarbonyl-[5-(3-aminoallyl)-2'- deoxyuridine 5'-triphosphate]

Flu-12-dUTP — Fluorescein-5(6)-carboxamidocaproyl-[5-(3-aminoallyl)-2'- deoxyuridine 5'-triphosphate]

Rho-5-dUTP — Tetramethylrhodamin-5(6)-[5-(3-carboxaminoallyl)-2'-deoxyuridine 5'-triphosphate

8-oxo-dGTP — 8-oxo-2'-deoxyguanosine-5'-triphosphate

ddNTPs — 2',3'-dideoxynucleoside-5'-triphosphates (ddATP, ddGTP, ddCTP and ddUTP)

All products are available as sodium, lithium, ammonium or triethylammonium salts.

Purity of dNTPs is 98 — 99 % according to HPLC analysis.

All product are free of DNases, RNases and phosphatases activities.

All product are free of pyroposphate.

All product are free of DNA and RNA.

dNTPs allows for synthesis DNA PCR-products up to 20 kilobases long, Real-Time PCR and other applications.

Custom Oligonucleotide Synthesis

Custom oligonucleotide synthesis in wide range of length, scale and different chemical modifications.

Enzymes from BIOSAN, Ltd.:

— DNA polymerase of the archaeon Pyrococcus furiosus

— M-MLV Reverse Transcriptase (RNase H Minus)

— T4 Polynucleotide Kinase

— T4 DNA Ligase

— T7 RNA Polymerase

— Taq DNA Polymerase

— Taqm DNA polymerase

— Thermostable DNA ligase of the archaeon Pyrococcus abyssi

DNA Ladders from BIOSAN, Ltd.:

- 100 bp DNA Ladder (400х2)

- 100 bp DNA Ladder (500х2)

- pBlueSK DNA/MspI Marker

- Lamda DNA/EcoRI+HindIII Marker

- 100+50 bp DNA Ladder

- DNA Ladder BX100-7

- 50 bp DNA Ladder

- 250 bp DNA Ladder

- 1 kb DNA Ladder

Menu of Reagents for PCR and Molecular Biology Research from BIOSAN, Ltd.:

2'-Deoxynucleoside-5'-triphosphates

- commonly used substrates for DNA synthesis;

- essential components for widespread molecular biology applications such as PCR for DNA research and medical diagnostics, DNA hybridisation probes production etc.

dATP - 2'-deoxyadenosine-5'-triphosphate

dCTP - 2'-deoxycytidine-5'-triphosphate

dGTP –2'-deoxyguanosine-5'-triphosphate

TTP - thymidine-5'-triphosphate

dUTP – 2'-deoxyuridine-5'-triphosphate

All products are available as sodium, lithium, ammonium or triethylammonium salts.

Quality of dNTPs:

Purity of dNTPs is 98 – 99 % according to HPLC analysis.

- All product are free of DNases, RNases and phosphatases activities.

- All product are free of pyroposphate.

- All product are free of DNA and RNA.

dNTPs allows for synthesis DNA PCR-products up to 20 kilobases long, Real-Time PCR and other applications.

Menu of Reagents for PCR and Molecular Biology Research from BIOSAN, Ltd.:

- 100 bp DNA Ladder (400х2)

- 100 bp DNA Ladder (500х2)

- pBlueSK DNA/MspI Marker

- Lamda DNA/EcoRI+HindIII Marker

- 100+50 bp DNA Ladder

- DNA Ladder BX100-7

- 50 bp DNA Ladder

- 250 bp DNA Ladder

- 1 kb DNA Ladder

Menu of Reagents for PCR and Molecular Biology Research from BIOSAN, Ltd.:

— DNA polymerase of the archaeon Pyrococcus furiosus

— M-MLV Reverse Transcriptase (RNase H Minus)

— T4 Polynucleotide Kinase

— T4 DNA Ligase

— T7 RNA Polymerase

— Taq DNA Polymerase

— Taqm DNA polymerase

— Thermostable DNA ligase of the archaeon Pyrococcus abyssi

Menu of Reagents for PCR and Molecular Biology Research from BIOSAN, Ltd.:

• AA-dUTP — 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate
• AA-UTP — 5-(3-aminoallyl)-2'-uridine 5'-triphosphate
• AZTTP — 3'-Azido-3'-deoxythymidine-5'-triphosphate
• Bio-11-dUTP — 5-[N-(N-biotinyl-Є-aminocaproyl)-3-aminoallyl]-2'-deoxyuridine-5'-triphosphate
• Br-dUTP — 5-Bromo-2'-deoxyuridine-5'-triphosphate
• 7-deaza-dGTP — 7-deaza-2'-deoxyguanosine-5'-triphosphate
• FITC-4-dUTP — Fluorescein-6-aminothiocarbonyl-[5-(3-aminoallyl)-2'- deoxyuridine 5'-triphosphate]
• Flu-12-dUTP — Fluorescein-5(6)-carboxamidocaproyl-[5-(3-aminoallyl)-2'- deoxyuridine 5'-triphosphate]
• Rho-5-dUTP — Tetramethylrhodamin-5(6)-[5-(3-carboxaminoallyl)-2'-deoxyuridine 5'-triphosphate
• 8-oxo-dGTP — 8-oxo-2'-deoxyguanosine-5'-triphosphate
• ddNTPs — 2',3'-dideoxynucleoside-5'-triphosphates (ddATP, ddGTP, ddCTP and ddUTP)

All products are available as sodium, lithium, ammonium or triethylammonium salts.

Purity of dNTPs is 98 — 99 % according to HPLC analysis.

All product are free of DNases, RNases and phosphatases activities.

All product are free of pyroposphate.

All product are free of DNA and RNA.

dNTPs allows for synthesis DNA PCR-products up to 20 kilobases long, Real-Time PCR and other applications.

Custom Oligonucleotide Synthesis:

Custom oligonucleotide synthesis in wide range of length, scale and different chemical modifications.

MBU has menu of ELISA kits for detection of hepatitis B markers in human sera or plasma.

Features and benefits:

 Kits provide all the necessary components to perform the test: The test-kits are fully completed with colour-coded reagents in order to prevent reagents confusing or mixing up.

 For more convenience – ready-to-use reagents format: The kits can be offered with ready-to-use conjugates and chromogen.

 For economy and workload flexibility – solid and stripped plates, in 96-well and 192-well formats: Use the required number of strips as new specimens are delivered to the laboratory.

 High sensitivity and specificity

 High reproducibility of results

 Reasonable price margin and reliable quality

 Test kits features:

 HBs-antigen-DS-strip confirmatory test kit is an indirect, competitive enzyme-linked immunosorbent assay intended to confirm the presence of HBsAg in human serum or plasma and combines direct and competitive ELISA assay for exclusion of false-positive results.

Incubation time: 2 h 20 min (80+40+20).

Shelf life: 12 months; plate: 3 months after opening.

 InVitroLogic-HBsAg is a sandwich-type enzyme-linked immunosorbent assay for the detection of HBsAg. Incubation time using shaker: sensitivity up to 0.05 IU/ml - 45 min (20+10+15) at 42 С or 1 h 5 min (30+20+15) at 37С; sensitivity up to 0.01 IU/ml - 1 h. 5 min (40+20+15) at 42 С or 1 h 45 min (60+30+15) at 37 С. Shelf life: 12 months; plate: 3 months after opening.

 InVitroLogic-anti-HBs is a sandwich-type enzyme-linked immunosorbent assay for the detection and quantitative determination of total anti-HBs. Incubation time: 1 h 20 min (40+20+20). Shelf life: 12 months; plate: 3 months after opening.

 InVitroLogic-anti-HBcore-IgM is an indirect solid phase enzyme-linked immunosorbent assay for the detection of anti-HBcore IgM. Incubation time: 1 h 15 min (30+30+15). Shelf life: 12 months; plate: 3 months after opening.

 InVitroLogic-anti-HBcore is an indirect solid phase enzyme-linked immunosorbent assay for the detection of total anti-HBcore. Incubation time: 1 h 10 min (60+10). Shelf life: 12 months; plate: 3 months after opening.

 InVitroLogic-anti-HBe is a sandwich-type enzyme-linked immunosorbent assay for the detection of total anti-HBe. Incubation time: 1 h 20 min (40+20+20). Shelf life: 12 months; plate: 3 months after opening.

 The products have the certification of country manufacturer (Russia) and have not CE-marking.

MBU has menu of ELISA kits for detection of HIV-infection markers: 3rd and 4th-generation HIV ELISA.

Features and benefits:

 Kits provide all the necessary components to perform the test: The test-kits are fully completed with colour-coded reagents in order to prevent reagents confusing or mixing up.

 For more convenience – ready-to-use reagents format: The kits can be offered with ready-to-use conjugates and chromogen.

 For economy and workload flexibility – solid and stripped plates, in 96-well and 192-well formats: Use the required number of strips as new specimens are delivered to the laboratory.

 High sensitivity and specificity

 High reproducibility of results

 Reasonable price margin and reliable quality

 Test kits features:

 InVitroLogic-HIV-1,2-Ab kit is 3rd generation HIV kits, an indirect solid phase enzyme-linked immunosorbent assay. Incubation time: 1 h 15 min (30+30+15). Shelf life: 12 months; plate: 3 months after opening.

 HIV-1,2-Ag/Ab kit is 4th generation HIV kit, a sandwich-type enzyme-linked immunosorbent assay. Incubation time: 1 h 55 min (60+30+25). Shelf life: 12 months; plate: 3 months after opening.

The products have the certification of country manufacturer (Russia) and have not CE-marking.

ELISA diagnostics of syphilis

ELISA sandwich-type test kits for the detection of total antibodies to Treponema pallidum in human serum or plasma.

Features and benefits:

 Kits provide all the necessary components to perform the test

 For more convenience - different colours of components in order to prevent components’ confusing or mixing up

 For economy - stripped plates: use the required number of wells as new specimens are delivered to the laboratory

 High sensitivity and specificity – the antigens of Treponema pallidum coating the wells of the plate are recombinant antigens

 High reproducibility of results

 Low price margin and reliable quality

 Updated one-stage ELISA test kit for the detection of total antibodies to Treponema pallidum in human serum or plasma including ready-to-use is available for customers:

InVitroLogic-Syph-Ab kit is a sandwich-type enzyme-linked immunosorbent assay. Incubation time using shaker: 30 min (20 +10); without shaker: 40 min (30+10). Shelf life: 12 months; plate: 3 months after opening.

The products have the certification of country-manufacturer (Russia) and have not Ce-marking.

ELISA diagnostics: STD test kits

MBU has a menu of traditional ELISA kits for detection of antibodies to microorganisms associated with Sexually Transmitted Diseases: Ureaplasma urealyticum, Trichomonas vaginalis, Mycoplasma hominis, Gardnerella vaginalis, Candida albicans.

Features and benefits:

 Kits provide all the necessary components to perform the test: STD test-kits are fully completed with colour-coded reagents in order to prevent reagents’ confusing or mixing up.

 Kits are compatible with most available equipment.

 For more convenience – ready-to-use component format: The kits are being offered with ready-to-use reagents, including Positive control, Negative control, Conjugate solution and Chromogen.

 For economy – stripped plates: use the required number of strips as new specimens are delivered to the laboratory.

 The simple uniform protocol of all STD test kits reduce technician errors.

 High reproducibility of results.

 Incubation time is 1 hour 20 min.

 Shelf life is 12 months.

 Reasonable price margin and reliable quality.

Menu of reagents for Immunoassays from BIOSAN, Ltd.:

Monoclonal antibodies (MAB) for diagnostics of tick-borne encephalitis virus (TBEV):

- MAB FV-5 (2H3)

Mouse MAB to envelope protein E of TBEV (strain Sofjin), IgM class, have neutralizing properties, complex-specific activity (i.e. react with all strain of TBEV and other viruses of TBEV complex), high association constant (Ka = 1,8 x 109 M-1).

- MAB FVN-22 (14D5)

Mouse MAB to envelope protein E of TBEV (strain Sofjin), IgG1 subclass, have neutralizing properties, partially complex specific activity (i.e. react with all strain of TBEV and other viruses of TBEV complex, except Langat virus ), high association constant (Ka = 1,8 x 109 M-1).

Menu of reagents for Immunoassays from BIOSAN, Ltd.:

- MAB HV-7: Mouse MAB for HBsAg (ay subtype), IgG class, specific for a determinant.

- MAB HV-11: Mouse MAB for HBsAg (ay subtype), IgG class, specific for y determinant.

- MAB HV-42: Mouse MAB for HBsAg (ad subtype), IgG class, specific for y determinant.

Monoclonal antibodies (MAB) for human immunoglobulins from BIOSAN, Ltd.:

MAB G5 are specific for kappa light chain of human immunoglobulins, belong to IgG class. MAB G5 labeled with horseradish peroxidase or other labels have been used as second antibody for diagnostics of various diseases, where the detection of specific antibodies is necessary.

HBs/HBc-Ag/Ab ELISA kit for the detection of HBsAg and/or total antibodies to HBcoreAg in human serum or plasma.

An upgraded sandwich-type HBV ELISA kit is designed by the company. The kit serves as a tool for diagnostics of HBV during the early stages of the infection and it allows to reduce diagnostic (seroconversion) window.

Features and benefits:

 HBV fourth-generation ELISA has been developed for simultaneous or separate detection of HBsAg and/or total antibodies to hepatitis B virus core antigen (anti-HBcAg) in human serum or plasma.

 High sensitivity and specificity: sensitivity level of detection of HBsAg – up to 0.05 IU/ml.

 High reproducibility of results

 Immunosorbent: Plate coated with a mixture of polyclonal antibodies to hepatitis B virus surface antigen HBsAg and recombinant hepatitis B virus core antigen (HBcAg).

 Conjugate 1 - Biotin conjugate: monoclonal antibodies to HBsAg conjugated with biotin, in buffer.

 Conjugate 2 - Streptavidin-HRP conjugate: streptavidin conjugated with horseradish peroxidase, in buffer.

 Conjugate 3 – Ag-HRP conjugate: recombinant hepatitis B virus core antigen (HBcAg) conjugated with horseradish peroxidase, in buffer.

 Incubation time using shaker: 1 h 20 min (40+20+20) at 42 С.

 Shelf life: 12 months; plate: 3 months after opening.

The product has not CE mark.

ELISA diagnostics of Chlamydiosis

ELISA kit for detection of antibodies of classes “A” and/or “G” to Chlamydia trachomatis in human serum or plasma: Comby-Chlamy-IgG/IgA-DS-Tr.

Features and benefits:

- Kit provides all the necessary components to perform the test

- For more convenience - each component is colored in its individual way in order to prevent components’ confusing or mixing up

- For economy - stripped plates: use the required number of wells as new specimens are delivered to the laboratory

-For task flexibility - kit composition permits to screen IgG and IgA separately or simultaneously

- For informative results - the analysis of IgG/IgA ratio in human serum or plasma allows to make an assumption about disease stage and choose an appropriate treatment

- High sensitivity and specificity

- High reproducibility of results

- Reasonable price margin and reliable quality

The products have the certification of country manufacturer (Russia) and have not CE-marking.

Control Panels are panels of human sera intended for use in external control and testing the work standards of clinical diagnostic laboratories. Control Panels are used to demonstrate serological lab proficiency.

Control Panels can also be used to challenge test kits performance, sensitivity, and specificity, to evaluate and validate new test methods, new equipment, show up the mistakes occurred during ELISA test kits performance, correct these mistakes, and train laboratory personnel.

Control Panels are included in the program of The Federal System External Quality Assessment (FSVOK) of Federal Service on Surveillance in Healthcare and Social Development of Russian Federation and local EQA programs. Each Control Panel contains 4 or 8 positive members and 1-4 negative members.

All sera are thoroughly characterized by a range of commercially available test kits. Qualification panels have similar intended use to that of the Control panels.

The sera included into the control panel are differentiated to positive and negative. The sera included into the qualification panel are also differentiated to positive and negative, and additionally positive sera are ranged in the order of marker concentration increasing.

Each Qualification Panel contains 4 positive members, which represent serial dilutions of human serum and 1 negative member.

Reference and Performance Panels are panels of human sera intended for use in control serological assays. Each panel consists of a thoroughly characterized series of serum collected from different individuals.

The sera members of panels represent the full reactivity range of the assay, including negative reactivity. Each panel also contains a comprehensive datasheet with testing results from a variety of commercially available test kits.

Reference and Performance Panels are used to challenge test kits performance, sensitivity, and specificity. Panels can be used to evaluate and validate new test methods, new equipment, demonstrate lab proficiency, and train laboratory personnel. As the panels represent the Russian collection of sera containing markers of infectious diseases and containing no such markers, they can be helpful for international users during for the test kits characteristics investigation.

These panels were developed and manufactured under the requirements specification of Tarasevich State Institute of Standardization and Control of Medical Biological Preparations. Each taken separately Reference Panel of sera was been recognized as Immunobiological Standard and has specific branch standard number(OCO).