[Oncology - Tissue biopsy] PNAClamp™ Mutation Detection Kit PIK3CA
PIK3CA mutation activates PI3K (Phosphatidylinositol 3-kinase) protein and PI3K activates several signaling pathways of tyrosine kinase receptors. Activated tyrosine kinase represses the function and excitometabolism of protein which inhibits cell survival and division. These abnormal changes initiate the process of cancer development by activating the protein involved in cell growth. Point mutations in PIK3CA are detected with ca. 80% of frequency in exon 9 (interpreted as helical part of PI3K protein) and exon 20 (interpreted as kinase part), while other types of mutations are also seen in many different locations. PIK3CA mutations are found with 25 to 40 % frequency in various types of cancer like colorectal cancer, gastric cancer, lung cancer, brain cancer, endometrial cancer, ovarian cancer, breast cancer, and more.
PNAClamp™ PIK3CA Mutation Detection Kit utilizes modified PCR technology, using optimized PNA probes that tightly bind to wild type DNA templates. This tight binding to the wild type DNA templates result in no amplification of the wild type DNA template in PCR reaction while the mutated genes, especially with SNPs, PCR amplification is processed for multiplication of the mutated DNA sequences.